Allelopathy Journal
  • Year: 2012
  • Volume: 30
  • Issue: 1

First isolation and identification of salicylate from Betula grossa var. ulmifolia - A potent root growth inhibitor

  • Author:
  • H. Araya1,, J. Otaka, E. Nishihara1,2, Y. Fujii1
  • Total Page Count: 6
  • Page Number: 153 to 158

1National Institute for Agro-Environmental Sciences, Kan-nondai 3-1-3, Tsukuba, Ibaraki, 305–8604, Japan

2Faculty of Agriculture, Tottori University, Kozancho-minami 4–101, Tottori, Tottori, 680–8550, Japan.

School of Agriculture, Meiji University, Higashimita 1-1-1, Tama-ku, Kawasaki, Kanagawa, 214–8571, Japan.

*Correspondence author: E.Mail: kinoko@meiji.ac.jp

Online published on 7 November, 2012.

Abstract

Isolation of salicylate from the bark of Betula grossa var. ulmifolia is reported first time. The identification was made by analysis of spectral data (1H, 13C NMR, EI-MS and FAB-MS) and direct comparison with an authentic standard sample following reversed phase HPLC analysis. The amount of salicylate in the dried bark was quantified as 0.031 mmol·g−1. The bark extract significantly inhibited the lettuce seedling root growth in 2-layer agar assay. The results indicated that B. grossa bark as litter could be important in allelopathy and salicylate may play part as allelochemical.

Keywords

Allelochemical, allelopathy, Betula grossa var. ulmifolia, litter, plant growth inhibitor, salicylate