Development of spectrophotometry methods for the determination of copper (II) Ions by new azo reagent based on Pyridine

The determination of macro-and micro quantities of metal ions by reagents, in manufactured chemical products, food and medicinal products, alloys, waste water and air is one of the most important problems facing the analytical services in their day-to-day work. Especially important is problem of determination of trace amounts of metals, ions, which have a number of unique properties and are increasingly used in various fields of science, technology, medicine and agriculture. The aim of the work was the elaboration of a new photometric method for determining copper (II) in the compositions of artificial mixtures, industrial alloys, natural waters and other objects. Photometric, spectrophometric, Asmus straight line method equilibrium shift and spectrophometric titration, Tolmachev method, Babko dilution method, additives method; the influence of number of factors on complex formation have been studied and optimal conditions for spectrophotometric determination of Cu(II) were found. The sensitivity of the new method has been determined (s_CuR2= 20000). The selectivity of the method was determined and the influence of outride ions was investigated. The developed spectrophotometry method was used to analysis of standard industrial samples (A-203-1, A-203-5, andM 123-1, M 99-5) and natural water ("Omonxona bulog'i") and obtained results were evaluated metrologically. In all cases relative standard deviation (S_r) doesn 't exceed 0.043.