Comparative study of the total protein and free amino acids of plants urtica dioica and crataegus pontica

The common proteins and amino acids of the Urticadioica plant and Crataeguspontica have been studied and compared. Stinging nettle raw material was collected during the flowering period in early June 2019 in the Bakhmal region. The raw material of Pontic hawthorn was collected during the flowering period in early May 2019 in the Gazalkent district of the Tashkent region. Total protein was determined by the Kjeldahl nitrogen method. The total protein content in the aerial part of the Urtica dioica L plant was 1.184%, and in the Crataegus L plant, 0.425%. The essence of the method consists in the decomposition of the organic matter of the sample with boiling concentrated sulfuric acid with the formation of ammonium salts, the conversion of ammonium into ammonia, its distillation into an acid solution, quantitative accounting of ammonia by the titrometric method and the calculation of the nitrogen content in the material under study. The amino acid composition was also studied; the synthesis of FTC (phenylthiocarbomail) derivatives of free amino acids was carried out according to the method of Steven A., Cohen Daviel.Results of the qualitative and quantitative determination of the amino acid composition. An aqueous extract of the Crataegus L plant contained 19 free amino acids with a total content of 0.25%, and in an alcoholic extract 20 amino acids with a total content of 0.73%. In an aqueous extract of the plant Urtica dioica L, 17 free amino acids with a total content of 0.086% were found, and in an alcoholic extract 17 amino acids with a total content of 0.196%. The content of free amino acids in leaves in alcoholic extracts of the Crataegus L plant is relatively predominant in alcoholic solutions, and the total content is 0.72%.