In vitro callus induction from embryos of matured seed explants of 11 rice varieties were selected for inducing callusing without and with different concentrations of NaCl (Salinity Stress). Observations on callus induction were carried out on callus induction MS medium having different concentration of hormones like 3 IAA, kinetin, 2,4-D and BAP with different concentrations 2.0, 0.5, 2.5 and 0.5 mg/l respectively. PH of the medium was adjusted to 5.8. Among different genotypes tested in the present study, CSR10, CSR13 and Jaya varieties showed better proliferation than CSR 30, Pokkali and CSR 27. The objective of the present study was to investigate the effect of genotype, growth regulator, and salt stress level on rice (Oryza sativa L.) callus induction. This study will be useful for selecting suitable callus induction medium for callus induction.
Callus Induction, Dehusked Rice, 2, 4-D, In Vitro, Rice
