Evaluation of Wound Biofilm Forming Bacterial Pathogens by Crystal Violet Binding Assay

In an abnormal environmental conditions or factors, the bacterial pathogens are force to colonize each other, either on abiotic surface/animal and plant tissues, thus forms a highly resistable structure called biofilm that are largely involving in multiple health issues while using colonized medical equipements. There is a challenge in bacterial pathogenesis to determine the degree of biofilm using an simple and effective way.

In order to explore such method and re-confirm the methodology, this study is designed to evaluate the wound biofilm producing bacteria by crystal violet binding assay.

The level of biofilm production was calculated by the stain absorbance on the surface of the test tubes.

As a result, among the isolates, the maximum of biofilm producer is Staphylococcus aureus followed by Pseudomonas aeruginosa.

The biofilm formation of the pathogenic bacterial species isolated from wounds showed the importance of understanding the adhesive nature of all the isolates. Further we planned to determine the usefulness of other stains in biofilm determination.