1Assistant Professor, Department of Biotechnology, Cauvery College for Women, Annamalainagar, Tiruchirapalli, Tamil Nadu, India Email id: ramyarbalaji@gmail.com
2Assistant Professor, Department of Biotechnology, Nehru Memorial College (Autonomous), Puthanampatti, Tamil Nadu, India
Polyhydroxybuty rates are biodegradable thermo polyesters which are synthesised by many bacteria under stress conditions they are reported to produce biopolymers, which can be produced intracellularly as carbon and energy reserves. In this present study, sixteen bacterial isolates isolated from sugar industry effluent collected from Sugar Industry, Perambalur. However, only one bacterial isolate (SAB4) is selected based on high PHB production 14.4±1.01mg/L. All the 12 bacterial isolates were characterised by morphological and biochemical test. Optimisation of culture conditions such as pH, temperature and incubation time was carried out for the isolate (SAB4). The maximum PHB yield was recorded under dry weight basis in minimal agar media as glucose as the sole carbon source at pH 7 and temperature 400C for 48 hours.
Polyhydroxy butyrate, Sago industry effluent
