1Department of Pharmaceutical Chemistry, Omega College of Pharmacy, Edulabad, Ghatkesar, Ranga Reddy Dist., Hyderabad-500 034
2Centre for Biotechnology, Department of Chemical Engineering, College of Engineering (A), Andhra University, Vishakapatnam – 530003
*Corresponding Author E-mail: satyadna_l@yahoo.co.in
Online published on 19 June, 2014.
A simple, precise, rapid and accurate reverse phase HPLC method was developed for the estimation of Raltigravir in tablet dosage form. A RP Inertsil ODS-3V C-18, 250x4.6 mm, 5!m partical size, with mobile phase consisting of 0.02M Dipotasium hydrogen orthophosphate in water pH 2.5 with orthophosphoric acid and Acetonitrile in the ratio of 60:40 v/v was used. The flow rate was 1.0 ml/min and the effluents were monitored at 210 nm. The retention time was 13.310 min. The detector response was linear in the concentration of 80–240μg/ml. The respective linear regression equation being Y= 56490.819X -302507. The limit of detection and limit of quantification was 0.2μg and 0.6μg/ml respectively. The percentage assay of Raltigravir was 98.86%. The method was validated by determining its accuracy, precision and system suitability.
The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Raltigravir in bulk drug and in its pharmaceutical dosage form.
Raltigravir, RP-HPLC, Estimation, and Tablets
