Asian Journal of Pharmaceutical Analysis

  • Year: 2020
  • Volume: 10
  • Issue: 3

UV spectrophotometric method development and validation of acotiamide in bulk and solid doage form

  • Author:
  • Vinod Matole, Avinash Birajdar, Suyash Ingle, Sagar Adlinge, Gajanand Nangare, Saili Madur, Smeeta Patil, Anita Shegaonkar
  • Total Page Count: 3
  • Page Number: 147 to 149

D.S.T.S Mandal's College of Pharmacy, Solapur-413004, Maharashtra, India

Abstract

Objective: A new, simple, sensitive, precise and reproducible UV spectroscopic method was developed for the estimation of Acotiamide in bulk and Tablet Formulation. Methods: The UV spectrum of Acotiamide in water showed λ max at 249 nm. Beer's law is valid in the concentration range of 3–18μg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 3–18μg/ml with regression equation y = 0.0523x - 0.0429 and regression correlation coefficient r2= 0.9996. Moreover, the method was found to be highly sensitive with LOD (0.38μg/ml) and LOQ (1.17μg/ml). Conclusion: Depending on results the given method can be successfully applied for assay of Acotiamide in Tablet formulation.

Keywords

Acotiamide, UV spectroscopy, Method development and validation, Water Tablet Formulation