Development and validation of stability indicating uplc method for the simultaneous estimation of drugs in combined dosage forms using quality by design approach

UPLC is a modern technique which gives a new direction for liquid chromatography. UPLC refers to ultra performance liquid chromatography, which enhance mainly in three areas: speed, resolution and sensitivity. Ultra performance liquid chromatography (UPLC) applicable for particle less than 2μm in diameter to acquire better resolution, speed, and sensitivity compared with high-performance liquid chromatography (HPLC). The concept of “Quality by Design” (QbD) is an approach which covers a better scientific understanding of critical process and product qualities, designing controls and tests based on the scientific limits of understanding during the development phase and using the knowledge obtained during the life-cycle of the product to work on a constant improvement environment.