Asian Journal of Pharmaceutical Analysis

In the presented work the Ultra-high Performance Liquid Chromatographic Time-of-flight Mass Spectrometric (UHPLC/Q-TOF-MS) method has been developed and validated for Simultaneous determination of Angiotensin Receptor Blockers (ARBs) Antihypertensive Drugs such as Losartan, Telmisartan, Valsartan, and Candesartan. The chromatographic separation was achieved on Acquity UPLCTM BEH C₁₈ (100.0 × 2.1mm, 1.7μm) column using isocratic mobile phase consisting of acetonitrile-2mM ammonium acetate (50:50, v/v) at a flow rate of 0.25mL/min. The Q-TOF mass spectrometer was operated in negative ionization mode and mass spectra were obtained by selecting the MS/MS transitions at m/z 421.20 to 127.00 for Losartan, m/z 513.16 to 469.13 for Telmisartan, m/z 434.50 to 255.50 for Valsartan, and m/z 439.10 to 309.90 for Candesartan. The fragmentation mechanism was established on the basis of mass spectra of drugs. The method was applied for pharmacokinetic study of these drugs in human plasma.