Asian Journal of Pharmaceutical Analysis

The analytical method was developed and validated for determination of acyclovir in Tablet by High performance liquid chromatography. The separation was carried out on Luna C₁₈ column (150*4.6mm, 5μm). The mobile phase consists of methanol: water in the ratio 50:50 at flow rate 1ml/min with diode array detector wavelength at 248 nm. The column temperature was adjusted at 38° ± 0.8±C with injection volume 20μl. The retention time of acyclovir was 2.718min. The linearity of the calibration curve was linear over the concentration range 40-60μg/ml (r²=1). The validation was carried out as per ICH guidelines. The development method was easy, rapid, linear, precise, accurate and consistent.