Development and validation of rp-hplc method for the estimation of acyclovir in API and tablet formulation

The analytical method was developed and validated for determination of acyclovir in Tablet by High performance liquid chromatography. The separation was carried out on Luna C₁₈ column (150*4.6mm, 5μm). The mobile phase consists of methanol: water in the ratio 50:50 at flow rate 1ml/min with diode array detector wavelength at 248 nm. The column temperature was adjusted at 38° ± 0.8±C with injection volume 20μl. The retention time of acyclovir was 2.718min. The linearity of the calibration curve was linear over the concentration range 40-60μg/ml (r²=1). The validation was carried out as per ICH guidelines. The development method was easy, rapid, linear, precise, accurate and consistent.