Method development and validation of empagliflozin in bulk and pharmaceutical dosage form using UV spectroscopy

A rapid and simple method of spectrophotometric estimation Empagliflozin in UV-region was developed and validated according to International Conference on Harmonization (ICH Q2 R1) guidelines. The standard and sample solutions were prepared using ethanol and water. The maximum absorbance (λmax) was obtained at 223 nm. The calibration curve was linear in the range of 1-30μg/ml. The %RSD for intra and inter-day precision was found to be 0.29 and 0.39 respectively. Recovery studies of Empagliflozin were within 99.20% to 99.49 and assay was found to be 99.87% and LOD and LOQ was found to be 0.10 and 0.33 respectively. The %RSD for robustness was found to be between 0.20 and %RSD for ruggedness was found to be 0.03. The proposed method was found to be rapid, precise, accurate and can be adaptable for routine analysis of Empagliflozin bulk and marketed dosage forms.