Analytical Perspectives on Clindamycin: Review of Methods in Formulations and Biological Samples

Strong antibiotics like clindamycin, which belong to the lincosamide class, are frequently used to treat bacterial infections, especially those that involve anaerobes and Gram-positive bacteria. The necessity for accurate, dependable, and effective analytical techniques for clindamycin measurement and quality control has grown as pharmaceutical companies work to comply with regulatory regulations. An extensive examination of the different analytical techniques created for the determination of clindamycin in pharmaceutical dosage forms, bulk medications, and biological matrices is presented in this article.The majority of the article is devoted to chromatographic techniques, particularly high-performance liquid chromatography (HPLC), which is still the most popular approach because of its exceptional resilience, accuracy, and precision. We evaluated several detection methods used in conjunction with HPLC, including UV, and fluorescence, as well as advancements in reversed-phase HPLC (RP-HPLC) for separation optimization. We examine alternative methods in addition to chromatography, such as spectrophotometric and electrochemical procedures, each of which has advantages specific to the matrix and analytical requirements. The selection of the type of column, the detection wavelength, and the mobile phase composition are only a few of the different factors that are addressed in depth while developing a method. The International Council for Harmonization (ICH) and other regulatory requirements are consulted for examining key validation criteria, such as linearity, accuracy, precision, limit of detection (LOD), and limit of quantification (LOQ).