1Department of Pharmaceutical Quality Assurance, NES’s Gangamail College of Pharmacy, Nagaon, Dhule, 424001, Maharashtra, India
2Associate Professor, Department of Pharmaceutical Quality Assurance, NES’s Gangamail College of Pharmacy, Nagaon, Dhule, 424001, Maharashtra, India
*Corresponding Author E-mail: nandinijadhav2503200@gmail.com
Online published on 5 March, 2026.
In this study, a Reverse Phase High-Performance Liquid Chromatography (RP-HPLC) method for the quantitative measurement of eutherosides in herbal formulations was developed and validated. Because of its exceptional sensitivity, resolution, and efficiency, RP-HPLC was selected. Using a Zodic C18 column and a mobile phase made up of acetonitrile and phosphate buffer (pH 8.5) in a 65:35 ratio, the technique used UV detection at 220 nm. According to ICH Q2A guidelines, the developed method was verified and showed excellent linearity (R2 = 0.9991) over a wide concentration range (500–3000 ng/spot), high accuracy (98–102% recovery), precision (RSD < 1.2%), and robustness under different pH and flow rate conditions. 9.89 μg and 29.98 μg were found to be the limits of detection and quantification, respectively. It turned out that the approach was precise, without external matrix components interfering. For the routine analysis of eluterosides in herbal pharmaceutical formulations, this validated RP-HPLC method is dependable and efficient.
RP-HPLC, Eleutheroside, Quantitative analysis, Validation, ICH guideline
