Development and Validation of Analytical Method for Estimation of Zinc and Manganese by Atomic Absorption Spectrophotometer in Multivitamin and Multimineral Syrup

This study aims to develop and validate analytical method for simultaneous estimation of Zinc and Manganese in Multivitamin and Multimineral Syrup by using Atomic Absorption Spectroscopy (AAS). Proper estimation of the content of Zinc and Manganese in the drug product is necessary to the confirm the quality of product. Multiple compendial and non compendial methods are available for estimation of minerals individually in different combinations of mineral products like Oil- and Water-Soluble Vitamins with Minerals Tablets, Oil- and Water-Soluble Vitamins with Minerals Capsules, Vitamins with Minerals Oral Powder, Oil- and Water-Soluble Vitamins with Minerals Oral Solution. No any compendial and non-compendial reference is found for simultaneous estimation of both Zinc and Manganese from Multivitamin and Multimineral Syrup. Perkin make Atomic absorption spectrophotometer is used. Instrument parameters, sample concentration and standard concentration were finalized by taking different trials. The method is validated as per ICH guidelines. The method applies for estimation of the content of Zinc and Manganese in formulation of Multivitamin and Multimineral Syrup. Simultaneous estimation of the minerals present in the product is necessary to save the time. Specificity, precision, linearity, accuracy, robustness and solution stability studies performed during validation study. The developed method found to be specific, precise, accurate, linear and robust. The correlation coefficient was 0.9989 for Zinc and 0.9996 for Manganese, the percentage relative standard deviation for precision study was found 1.28 for Zinc and 0.66 for Manganese, the mean recovery was found to be well within limits 90 to 115%.