Contemporary Trends in Analytical Method Development for Dapagliflozin and Pioglitazone: A Critical Review of Spectroscopic and Chromatographic Approaches

This review gives an overall overview of the different analytical methods available to determine dapagliflozin and pioglitazone, which are used to treat people suffering from type 2 Diabetes Mellitus. Accurate measurement of these two drugs is essential for testing their effect on humans in many ways; they can there for help researchers understand their safety profiles and help ensure that all drugs sold as medicine meet certain criteria (Quality Control). There are many different analytical chemistries available to measure dapagliflozin and pioglitazone in bulk and pharmaceutical preparations, as well as in human biological materials. Evaluating the various analytical methods available, we are specifically referring to methods of validation: For example, when evaluating an absolute measure of dapagliflozin and pioglitazone, we would evaluate the validity of the method by determining its precision, and Accuracy; therefore, this method would also be called Relative Precision and Relative Accuracy, using the validated dose concentration. Analytical methods are generally considered to be highly specific, therefore creating strong, reliable and reproducible analytical method should be selected based on the needs regarding accuracy, reliability, and cost. Some examples of analytical methods that could be used would include RP-HPLC, HPTLC, UV Spectroscopy, and Stability Testing, along with the new developments being made in analytical chemistry techniques.