UV Spectrophotometric Simultaneous Determination of Metformine Hydrochloride and Pioglitazone Hydrochloride in Combined Dosage Form

Simple, sensitive, specific and economic spectrophotometric method was developed and validated for simultaneous quantitation of Metformine Hydrochloride and Pioglitazone Hydrochloride in tablet dosage form. New method based on the simultaneous estimation of drugs in a binary mixture without previous separation was developed. In simultaneous equation method, Metformine Hydrochloride and Pioglitazone Hydrochloride were quantified using their absorptivity values of at selected wavelengths, viz., 233 nm and 265.5 nm respectively. The accuracy and reproducibility of the proposed method was statistically validated by recovery studies. The simultaneous equation method permits simple, rapid and direct determination of Metformine Hydrochloride and Pioglitazone Hydrochloride in commercially available tablet dosage form without previous separations and can therefore be used for routine analysis of both drugs in quality control laboratories.