1Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazige University, Zagazig, Egypt
2Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Al –Azhar University, Assuit, Egypt
Two spectrophotometric methods are described for determination of Cefepime, Cefoperasone and Cefotriaxone in bulk and pharmaceutical dosage forms using insitu generated bromine as oxidizing agent and either methylene blue or methyl orange as chromogenic agents. Drugs are treated with known excess of bromine and residual unreacted bromine is determined by treating with fixed amount of either methylene blue or methyl orange then measuring absorbances at 678 nm and 510 nm, respectively. The amount of bromine reacted corresponds to the amount of each drug. Effect of acidity, bromate -bromide volume and reactiontime, on the absorption was studied. Calibration curves were linear over ranges of 1–3 μg.ml−1 for Cefepime,0.4–1.0 μg.ml−1 for Cefoperazone and 0.3–0.8 μg.ml−1 for Cefotriaxone in case of methylene blue and of 0.05–3.0 μg.ml−1 for Cefepime, 0.75–2.0μg.ml−1 for Cefoperazone and 0.2–1.4 μg.ml−1 for Cefotriaxone in case of methyl orange. The methods were satisfactory applied for the determination of drugs in both bulk and pharmaceutical forms and results were compared statistically with reference methods.
Cefepime, Cefoperazone, Cefotriaxone, Methylene blue, Methyl orange
