Department of Medicinal Chemistry, Faculty of Pharmacy, Zagazige University, Zagazig, Egypt
Online published on 5 February, 2015.
An isocratic HPLC method had been developed for rapid simultaneous determinathion of three cephalosporines including cefepime HCl, cefoperazone, and cefotaxime Na in pure form or in pharmaceutical dosage form within less than 15 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) using a mobile phase of MeOH: 0.025M KH2PO4 adjusted to pH 7.8 using triethyalamine (18:82 v/v) at ambient temperature. The flow rate was 1 ml/min and maximum absorption was measured at 254 nm.
Cefepime, Cfotaxime, Cefoperazone, Phosphate buffer and HPLC
