UV Spectrophotometric Method Development and Validation for the Estimation of Gliclazide in Bulk and Pharmaceutical Dosage Form

A simple, sensitive, rapid, accurate, reproducible and less time consuming visible spectroscopic method has been developed for the determination of Gliclazide in pure and tablet dosage form. The wavelength maxima for gliclazide was found to be 224.05nm. Beer's law was obeyed in the concentration range of 2–20μg/ml. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.15μg/ml and 0.50μg/ml. The percentage recovery of the drug for the proposed method was 99.29% indicating no interference of tablet excipients. The results demonstrate that proposed method is accurate, precise and reproducible while being simple and rapid too for determination of gliclazide in tablet dosage form.