Method Development and Validation for Simultaneous Estimation of Omeprazole and Ofloxacin by using RP-HPLC

A simple, Accurate, precise Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) method was developed for the simultaneous estimation of the omeprazole and Ofloxacin in Tablet dosage form. Chromatogram was run through ODS (150X4.6mm, 5μ). Mobile phase containing Buffer and Acetonitrile in the ratio of 45: 55 was pumped through column at a flow rate of 0.8 ml/min. Buffer temperature was maintained at 30°C. Optimized wavelength for Omeprazole and Ofloxacin was 220nm. Retention time of Omeprazole and Ofloxacin were found to be 2.16 min and 3.39 min. %RSD of the Omeprazole and Ofloxacin were and found to be 0.62 and 0.74 respectively. %Recover was Obtained as 100.07% and 100.72% for Omeprazole and Ofloxacin. LOD, LOQ values were obtained from regression equations of Omeprazole and Ofloxacin were 0.27ppm, 0.37ppm and 0.83ppm, 1.13ppm respectively. Regression equation of Omeprazole is y=11878x+281.6, and of Ofloxacin is y=14453x+3910.2.