Ecofriendly Validated Spectrophotometric Method for the Estimation of Amlodipine Besylate by using Hydrotropic Solubilization Method

Simple sensitive and accurate spectrophotometric methods were developed for determination of poorly water soluble Amlodipine Besylate in pure and pharmaceutical dosage form using hydrotropic agents ammonium acetate. Hydrotropy is a good choice for replacing organic solvents used for this kind of drugs to reduce the cost and hazards of the analytical method. Amlodipine Besylate shows maximum absorption at 362 nm in 0.5 M of the hydrotropic agents. Beer's law was found to be obeyed in the concentration range of 5–25 μg/mL for the hydrotrope. Limit of detection was found to be 1.102μg/mL and limit of quantification was 3.278 μg/mL for ammonium acetate. The results were in a good agreement with those obtained with official USP method.