Development and Validation of UV Spectrophotometric Method for Estimation of Pregabalin

In the present work simple fast, accurate, precise and robust method for analysis of pregabalin using derivatizing agent is reported. Method is simple as it requires no tedious sample preparation and hence can be used for routine analysis of pregabalin. The maximum absorbance of the pregabalin was found to be 210nm. The maximum UV absorption of the derivatized pregabalin was determined at 226nm. The given method was found to be linear in the concentration range 01–20 μg/mL. The performance of the develop method was evaluated in terms of standard deviation and relative standard deviation to find out the significance of proposed methods over the reference spectrophotometric method. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also precise.