Asian Journal of Pharmaceutical Analysis

A simple, accurate and precise spectrophotometric method including area under curve has been developed for the estimation of Deferiprone (DEF) in bulk material and capsule dosage form. Deferiprone is an iron chelating agent, used in the treatment of thalassemia syndromes when iron overload due to blood transfusions occurs. Four simple UV Spectrophotometric methods were established for determination of Deferiprone, using double beam UV spectrophotometer (UV-2450, Shimadzu, Japan). Deferiprone has absorbance maxima at 278 nm with distilled water used as a solvent. Deferiprone obeys Beer-Lambert's law in the concentration range of 2–10 μg/mL. The % recovery by proposed methods was found to be 98–101%. The Precision studies show less than 2 in terms of %RSD indicates precise nature of proposed method. All methods were validated as per the ICH guidelines, Q2 (R1). The statistical data and results prove that developed methods are simple, accurate and precise and can be used for the routine analysis of DEF in bulk and pharmaceutical formulation.