Optimization of Drug loading in Modified Nano-zeolites using response surface Methodology by Box-Behnken Design

Nano-zeolite NaA and cancrinite were synthesized from natural iranian clinoptilolite and Nano-zeolite sodalite was synthesized from volcanic glass perlite as silica sources. Sodium aluminate was used as aluminium source. The synthesized Nano-zeolites were characterized about structure and morphology To improve drug loading capacity of the carrier, all the synthesized Nano-zeolites were modified with Cetyl trimethyl ammonium bromide Response surface methodology by employing a 3-factor, 3-level Box-Behnken design was used to optimize three parameters, i.e. the type of zeolites, pH, and time for adsorption of Diclofenac sodium. Characterization methods confirmed the structure of Nano-zeolites. Drug concentrations were investigated at max of 275nm by Ultra Violet-Visible spectrophotometer. The Optimum conditions for drug loading were obtained pH=4, time for adsorption of 120 min and cancrinite as the best modified Nano-zeolite In-vitro release studies were performed at optimum condition of adsorption, at simulated pH and temperature of in Vivo condition. Results show that the release of drug is increased with increasing in pH and time. Therefore, drug release will be increased when the nanoparticles move down the gastrointestinal tract from the stomach to the intestine. A desirable controlled release for drug and an operative carrier for oral drug delivery were introduced. Applying clinoptilolite and perlite as silica sources is cost-effective and available. Using response surface methodology (RSM) by employing a 3-factor, 3-level Box-Behnken design (BBD) is an effective tool for optimization of the adsorption of diclofenac sodium (DS) onto modified nanozeolites as drug carriers. Synthesized carrier can control releasing of the drug in body and enhance therapeutic efficacy of drug.