Study of The effect of L-Riboce-D-Cystein on X-Ray-Induced Testiculur Damage in Adult Wistar Rats

This research investigated the effect of D-Riboce-L-cysteine on X–ray induced testicular injury in adult Wistar rat. Twenty male rats were split into five (5) groups of four (4) animals at random. Group 1 consisted of control animals that were given only regular food and drink. Only X-rays were used to induce Group 2, these were administered at approximately 95 kV, 12.5 mA/s, and 50 focal field distances (FFD) per animal, primarily in the pelvic and perineum regions. Group 3 underwent treatment with 30 mg/kg body weight with D-Riboce-L-cysteine, followed by an X-ray treatment at 95 kV, 12.5 mA.s, and 50 FFD each animal. Before receiving 30 mg/kg body weight of D-ribose-L-cysteine, Group 4 was treated with 95 kv, 12.5 milliampere-seconds (mA.s), and 50 FFD of X-ray each animal. Only 30 mg/kg body weight of D-ribose-L-cysteine was administered to Group 5. Following a 21-day course of treatment, the animals were sacrificed, the testes were removed and preserved in Bouins fluids for histological examination, and the right testis was homogenized in 5% sucrose solution to measure tissue malonialdehyde (MDA) and the activity of glucose-6-phosphate dehydrogenase (G-6-PDH) and glutathione peroxidase (GSH-P). The caudal portion of the epididymis was immersed in a standard saline solution to assess sperm motility, count, and morphology. The animals receiving no treatments showed significantly greater sperm count, sperm motility and sperm morphology when compared with the treated groups (Group 2,3 and 4) at (p>0.05) and minor differences found when compared with group 5 (D-Riboce-L-cysteine alone) at (p>0.05). The control group exhibited a statistically significant increase in G-6-PDH activity in relation to all other groups (p>0.05). The control group had lower MDA levels than all other groups, with the exception of group 5 (antioxidant alone), which had marginally higher MDA levels (p>0.05). In contrast, both the control group and the animals receiving D-Ribocine-L-cysteine group. The testicular lobules, seminiferous epithelium, and its constituent cells are all visible in the photomicrograph of the testis from the animals in group 1 (Control), which displays normal testicular histology. The animals in group 2 have a wider interstitial gap, a loss of basal laminal tissue, and spermatogonia production following vaculation in the photomicrograph (X-ray only). There was also evidence of seminiferous tubule germinal epithelium loss. The photomicrograph of the testis of the animals in groups 3 and 4 (D-Riboce-L-cysteine+X-ray) revealed aberrant interstitial space expansion, decreased leydig cell, and spermatogonial cell degeneration, all of which indicate vaculation. In Conclusion, D-ribose-L-cysteine seems to has a significant impact on preventing testicular damage caused by X-rays. This suggests that D-Riboce-L-cysteine has the potential to enhance fertility.