Estimation of Quercetin in Mangifera indica Leave Extract by Planer Chromatographic Method

Mangifera indica L. belongs to family Anacardiaceae is an important herb in ayurvedic and indigenous medical systems for over 4000 to treat numbers of diseases like Diabetes, Dissolve gall and kidney stones, cures respiratory problems, treat throat problems, treat restlessness, treat hiccups, helps to lose weight and also heals burns. A sensitive, simple, and accurate High Performance Thin Layer Chromatographic method has been used for quantitation of Quercetin from of Mango Leaf powder. Phytochemical Screening was performed to identify various chemical constituents present in the plant. The chromatographic development was done on HPTLC plates recoated with Silica Gel 60 F₂₅₄ utilizing a mobile phase of Toluene: Ethyl Acetate: Formic acid (4: 5: 1 %v/v/v) as flexible stage. The eluted analytes detected at 374 nm. Phytochemical screening gives promising data for presence of various chemical constituents like, Alkaloids, Flavonoids, Glycosides, Steroids, Glycosides, Proteins, Tannins and Phenolic Compounds, Volatile oil etc. In developed HPTLC method quercetin was found at 0.84 R_f as compared with standard Quercetin. Present study confirms the presence of various chemical constituents in the leaf of Mangifera indica. Also, it confirms the presence of Quercetin in different extract of Mangifera indica leaves. Highest concentration of Quercetin was found in ethyl acetate extract where lowest amount was found in ether extract.