Influence of Incubation Temperatures on Total Phenolic, Flavonoids Content and Free Radical Scavenging Activity of Callus from Heliotropium indicum L.

The incubation temperatures such as 20, 25, 30 and 32°C were used on callus culture of Heliotropium indicum L. for assessment of total phenolic, flavonoids content and free radical scavenging activity. The callus biomass was decreased in the treatments than the control (1.92 ± 0.01 g/tube). The texture of the callus (compact) was same in all the treatments but the colour was slightly different. The highest amount (10.29 ± 0.09 mg/g) of total phenolic content was estimated from callus grown under 30°C. Callus from the same treatment shows maximum percentage (53.17 ± 1.43) of free radical scavenging activity.