Alternative exploration of hyaluronic acid from marine superstore

Hyaluronic acid (HA) is non-sulphated, linear glycosaminoglycans (GAGs) extensively used in biomedical, cosmetic and nutraceutical field. This type of GAGs has created a more attraction to biologists for exploration of HA from various sources. Consequently, this present study was focused towards the marine mollusk for alternative source target for the isolation of HA from the gastropod-Hemifusus cochlidium. The whole body tissues were defatted by acetone and pellet was extracted using digestion buffer followed by proteolytic treatment. Then the crude GAGs were subjected to anion exchange column for purification. Further, HA, D-glucuronic acid and N-acetyl glucosamine content was measured using the calorimetric method. Fourier transform infrared spectroscopy (FTIR) and Nuclear magnetic resonance (H¹ NMR) were used for characterization. The yield from H. cochlidium and the presence of functional group in the isolated HA was characterized by (FTIR) and H¹ NMR. In this investigation, the results suggested that marine gastropod could be an alternative source of HA.