An isocratic HPLC method had been developed for rapid simultaneous separation and determination of two cephalosporins with one macrolide including Claritromycin, Cefixime and Cefoperazone in pure form or in pharmaceutical formulations within less than 5 minutes. Separation was carried out on a Hypersil gold C18 (10um, 100x4.6mm) column. Effect of pH and composition of mobile phase in addition to flow rates was studied. Beer's law was obeyed in the range of 1–50 μg/ml for Clarithromycin and Cefixime or 2–50 ug\ml for Cefoperazone. The method was applied for the determination of drugs in both bulk and pharmaceutical forms and were validated when obtained results were compared with reference methods.
Clarithromycin, Cefixime, Cefoperazone, Phosphate buffer and HPLC
