Absorbance Ratio Method of Vortioxetine and Aripiprazole in Synthetic Mixture by UV Spectrophotometry

The absorbance ratio method is a modification of the simultaneous equations procedure. In this study, absorbance ratio analytical method is developed in UV spectrophotometry of Vortioxetine and Aripiprazole in synthetic mixture. The measurements were carried out at wavelengths of 225 and 255 nm. The 255nm is the λmax of Aripiprazole & 225nm is the isobestic point. The linear correlation of Isobestic point (r²=0.997) was obtained in the range of 2-20 μg/ml and for Aripiprazole the linear correlation range (r²=0.998) was obtained in the range of 2-32 μg/ml. The method was successfully used for absorbance ratio method of Vortioxetine and Aripiprazole in synthetic mixture form without any interference from excipients and prior separation.