Phytochemical Profiling, Anticancer and Thrombolytic activity of Limonia acidissima L. leaf

Limonia acidissima L. is one of the medicinal plant, which belong to Rutaceae family. The indigenous system of medicine suggests the use of all parts to treat various ailments. The purpose of this study was to identify the presence of secondary metabolites and to investigate anticancer and thrombolytic activity of hydroalcoholic extracts of Limonia acidissima. Leaf samples of Limonia acidissima were gathered from the local communities in Akluj, Solapur, Maharashtra, India. The leaf were dried, powdered, and extracted with hydroalcoholic using a cold maceration method. Standard chemical tests were performed to determine the presence of secondary metabolites in the Limonia acidissima leaf extract. The Lethality Assay for Brine Shrimp was used to determine the cytotoxic potential of leaf extracts. Thrombolytic activity was evaluated by clot lysis. The results obtained revealed that the hydroalcoholic extract and the standard of 5 fluorouracil LC₅₀ values for anti-cancer activity were 40 and 32.21µg/ml, respectively. The thrombolytic activity of the hydroalcoholic extract and aspirin standard had IC₅₀ values of 202.9 and 67.7721µg/ml, respectively. Plant’s leaf might be used for the production of new anticancer and thrombolytic drugs.