Preparation of nanobiocatalysts with the combination of nanotechnology and biotechnology is considered as an exciting and rapidly emerging area. The use of iron oxide magnetic nanoparticles as enzyme immobilization carriers has drawn great attention because of their unique properties, such as controllable particle size, large surface area, modifiable surface, and easy recovery. In this paper, a variety of surface modification on synthesized magnetic iron oxide by different ways and a variety of magnetic iron oxide nanoparticles immobilized enzymes in bioprocess applications, especially enzymes like lipase and cellulase are reviewed. The advantages and shortcomings of the magnetic iron oxide nanoparticles immobilized enzymes and the future developing prospects are also discussed.
