1Final Year, Department of Biotechnology, Sri Venkateswara College of Engineering, Sriperumbudur
2Assistant Professor, Department of Biotechnology, Sri Venkateswara College of Engineering, Sriperumbudur
Online published on 5 June, 2014.
In situ hybridization (ISH) is an efficient method for detecting the localization of mRNA. It has a lot of disadvantages that it doesn't provide information about the time dependent analysis also the information of post translational modification. They also suffer from serious disadvantage that they does not allow mRNA detection in living cells. Care should be taken in the extraction of the biomolecules from living cells that severe damage to the cells should be avoided. This review reveals the development of nano-structured oligonucleotide probes that has a predominant role in the detection of mRNA in the living cells. In particular, we describe methods that emphasize on nanotechnology in diagnosis and treatment for cancer.
