1Research Scholar, Department of Pharmaceutical Analysis, Jawaharlal Nehru Technological University, Kakinada, Kakinada -533 003-Andhra Pradesh, India
2Department of Pharmaceutical analysis and QA, Teegala Ram Reddy College of Pharmacy, Meerpet, Hyderabad-500097. Telangana
3Chaithanya College of Pharmacy, Markapur, Prakasham (Dist). A.P., India
4Chilkur Balaji College of Pharmacy, Aziz Nagar, Hyderabad, Telangana
*Corresponding Author E-mail: Kumaraswamy.gandla@gmail.com
Online published on 5 February, 2015.
The present work deals with the development of a precise, accurate, simple, specific, reliable and less time consuming RP-HPLC method for the estimation of Pseudoephedrine and Terfinadine tablets. The chromatographic separation was achieved on a Inertsil C18 ODS (4.6 x 250mm, 5μm) with a mobile phase combination of methanol and water (50:50) v/v at a flow rate of 1.0 ml/min, and the detection was carried out by using PDA detector at 290 nm. Ambient column temperature has maintained. The total run time was 10mins. The retention time of Pseudoephedrine and Terfinadine were found to be 2.9 min. and 3.5 min. respectively. The performance of the method was validated according to the present ICH guidelines.
RP-HPLC Method, Pseudoephedrine and Terfinadine, tablet dosage forms, PDA Detection
