Absorption Correction Method for Estimation of Nebivolol and Hydrochlorothiazide in Combined Tablet Dosage Form

A new, simple, accurate and sensitive UV-spectrophotometric absorption correction method has been developed for simultaneous determination of Nebivolol and Hydrochlorothiazide in combined pharmaceutical dosage form. The method is based upon determination of Nebivolol at 281 nm and Hydrochlorothiazide at 316.5 nm, in aqueous methanol (20% v/v). Nebivolol hydrochloride and Hydrochlorothiazide at their respective λ_max 281.0 nm and 316.5 nm shows linearity in the concentration range of 5–35 μg/ml and 10–70 μg/ml respectively. The method was validated statistically.