Validation of UV spectrophotometric and HPLC methods for quantitative determination of imatinib in bulk and pharmaceutical dosage form

The main objectives of presented work were developed and validate the UV-spectrophotometric and RP-HPLC method for the estimation Imatinib as per ICH guidelines in bulk and pharmaceutical dosage form. In UVspectrophotometric and HPLC method was developed by using solvent Acetonitrile and water in the ratio of 50: 50 (%v/v). The RP-HPLC method was developed in Symmetry C₁₈(4.6 × 150mm, 5μm). In UVspectrophotometric method, the optimal response for two of them was obtained at 242 nm. Hence, the λmax of UV and HPLC for Imatinib was 242 nm were found to be respectively. It was proved that linearity in the concentration range for Imatinib was 0.5–3 μg/ml and for HPLC the linearity range between 0.25–1.5μg/ml for Imatinib 0.5–3 μg/ml with a correlation coefficient value of 0.999. The % RSD for intraday precision and interday precision was less than 2% for UV-spectrophotometric and RP-HPLC method. A new, simple, economic and accurate UV-spectrophotometric and RP-HPLC method were developed for the estimation of Imatinib in the bulk and in the pharmaceutical dosage form.