Central Research Laboratory, D. G. Ruparel College, Matunga, Mumbai-400016
A novel reverse phase liquid chromatographic method was developed and validated for estimation of perinatal HIV-1 reverse transcriptase drug, emtricitabine which also active against Hepatitis B virus in its dosage form i.e. tablets. The reverse phase HPLC analysis was carried out with isocratic system. The column was Peerless basic C18 (50mm x 4.6mm, 3μm) with ambient temperature. The mobile phase consisted of buffer: methanol in proportion 90:10% (v/v). The flow rate was maintained at 1 ml/min. The detection was carried out at wavelength 280 nm. The method was validated as per ICH guidelines for system suitability, linearity, accuracy and precision. The linear ranges were 50–150 μg/ml for emtricitabine. The accuracy and precision were found to be well within the acceptable limit. The method was successfully applied for determination emtricitabine in dosage form with good recoveries.
Emtricitabine, Tri ethyl amine, Phosphoric acid, Methanol, HPLC
