Asian Journal of Research in Chemistry

  • Year: 2020
  • Volume: 13
  • Issue: 3

Development of Validation of Zero order, First-order, Second-order, UV-Spectrophotometry Methods using AUC Technique for Quantitative Estimation of Linagliptin in Bulk Material and Tablets

1R.C. Patel Institute of Pharmaceutical Education and Research, Shirpur (MS) 425405 India

2Ahinsa Institute of Pharmacy, Dondaicha, Shindkheda, Dhule. (MS) 425408 India

Abstract

The present study deals with development and validation of a simple, rapid, sensitive and economic UV Spectrophotometry method Using AUC technique for Quantitative estimation of Linagliptin bulk material and tablet dosage form.

In method A, B and Ca stock standard solution was prepared by dissolving 10 mg of Linagliptin in 100 mL of 10% v/v methanol to obtain a concentration of 100 μg/mL. After suitable dilution, 10 μg/mL of Linagliptin was prepared and scanned in the UV-visible range 400–200 nm; Linagliptin showed a maximum absorbance at 295 nm. In Method A, Area Under Curve, (Auc)of the zero-order spectrum was recorded between 280 and 307 nm. While, in Method B, C, zero-order spectra were derivatized into first and second-order, and the AUC was recorded between 302 and 317 nm, and 290 and 303 nm,

In Method A, B and C, linagliptin followed linearity in the concentration range of 4–24 μg/mL (r2>0.999).

The amount of drug estimated for this methods are in good accord with labeled claim. The % RSD value in recovery, precision, and ruggedness studies are found to be not more than 2 indicated that the method is accurate and precise.

Keywords

Linagliptin, U.V Spectroscopy, Derivative, Methods, Area Uder Curve, (AUC)