Asian Journal Of Research in Chemistry

  • Year: 2021
  • Volume: 14
  • Issue: 1

Total phenolic contents and determination of Antioxidant activity by DPPH, FRAP, and cyclic voltammetry of the fruit of Solanum nigrum (Black nightshade) growing in the south of Algeria

  • Author:
  • Imene Guediri1,4,*, Cherifa Boubekri2,4,*, Ouanissa Smara1,5,*, Touhami Lanez3,4,*
  • Total Page Count: 9
  • Published Online: Apr 15, 2021
  • Page Number: 1 to 9

1University Kasdi Merbah, Faculty of Material Science, Department of Chemistry, Ghardaia BP Road 511. 30,000, Ouargla, Algeria

4University of El Oued, VTRS LaboratoryB.P.789, 39000, El Oued, Algeria

2University Mohamed Khider, Faculty of Exact sciences, Natural and Lile Sciences, Department of Material sciences, BP145RPBiskra07000Algeria.

5Laboratory Valorisation et Promotion des Ressources Sahariennes (VPRS), 30000, Ouargla, Algeria

3University of Echahid Hamma Lakhdar, Faculty of Exact sciences, PO Box 789, 39000, El Oued, Algeria

Abstract

In the present research, we studied the quantification of the total phenolics, total flavonoids, total flavonols, and the evaluation of the antioxidant activity of the fruits of Solanum nigrum L. by chemical and electrochemical methods. The chloroform, ethyl acetate, and n-butanol crude extracts are prepared from the hydroethanolic extract of the fruits were tested. The results showed that ethyl acetate extract showed the highest phenolic content (87.68mg GAE/g), chloroform extract showed the highest flavonoid content (24.23mg QE/g) and highest flavonol content (71.60mg RE/g). The evaluation of antioxidant activity by chemical methods revealed that the highest DPPH radical scavenging activity was shown by ethyl acetate extract (0.123mg/mL) while the highest ferric reducing capacity was shown by butanol extract (610.46±0.0015mg FeSO4/g). The electrochemical study of the three extracts by cyclic voltammetry method indicates that the highest antioxidant activity was shown by ethyl acetate extract (17.75±0.238mg/g) equivalent ascorbic acid. The superoxide scavenging assay of chloroform extract showed the highest value (0.8 mg/mL).

Keywords

Solanum nigrum L, Fruit extraction, Phenolic profiles, Antioxidant activity, Cyclic voltammetry, Superoxide anion radical