1Faculty of Technology, University Amar Telidji-Laghouat, P.O. Box 37G, Laghouat, 03000, Algeria
10Renewable Energy Development unit in Arid Zones (UDERZA), University of Echahid Hamma, Lakhdar, El Oued, 39000, Algeria
2Laboratory of Management and Valorization of Natural Resources and Quality Assurance, SNVST Faculty, University of Bouira, Bouira, 10000, Algeria
4Department of Chemistry, Faculty of Mathematics and Material Science, University Kasdi Merbah Ouargla, P.O. Box 511, Ouargla, 30000, Algeria
5Chemical Engineering Department, College of Engineering, University of Ha'il, P.O. Box 2440, Ha'il, 81441, Saudi Arabia
6Chemical Engineering Process Department, National School of Engineering Gabes, University of Gabes, Gabes, 6011, Tunisia
7Chemical Engineering Department, Faculty of Engineering, University of Blida, P.O. Box 270, Blida, 09000, Algeria
8Laboratory of Industrial Analysis and Materials Engineering (LAIGM), University 8 May 1945 Guelma, BP 401, Guelma, 24000, Algeria
9Department of Process Engineering and Petrochemical, Faculty of Technology, University of Echahid Hamma, Lakhdar, El Oued, 39000, Algeria
*Corresponding Author E-mail: bbachir39@gmail.com
Online published on 19 February, 2022.
Plants are a large source of new bioactive molecules with therapeutic potentials. However, only a small amount of worldwide plants has been phytochemically investigated. The ethanolic extracts from leaves and flowers of Paronychia argentea were evaluated for their antilithiasic activity in vitro. The effect of extract (0.1, 0.2, 0.3, 1, 2, and 5mg/mL) was studied by the measurement of turbidity in presence or absence of extract at 620nm using UV/Vis spectrophotometer. Total phenol and flavonoid contents were also evaluated. Polyphenol content was found to be more present in the leaves extract (9.29±0.009mg of Gallic acid equivalent (GAE)/g) compared to the flowers extract (5.92±0.14mg GAE/g). Flavonoids content was also found to be more present in the floral extract thatis estimated at 0.18±0.01 mg QE/g compared to the flowers extract (0.47±0.0035mg QE/g). For the antilithiasis activity, the results clearly shown that P. argentia extracts inhibited calcium oxalate crystallization by concentration-dependent manner. The maximum percent inhibition of calcium oxalate by flowers extract was found to be 70.97% at 5mg/mL. Further, P. argentea leaf extract has shown antilithiasic properties and may be used for the prevention of kidneys stones. Plants are a large source of new bioactive molecules with therapeutic potentials. However, only a small amount of worldwide plants has been phytochemically investigated. The ethanolic extracts from leaves and flowers of Paronychia argentea were evaluated for their antilithiasic activity in vitro. The effect of extract (0.1, 0.2, 0.3, 1, 2, and 5mg/mL) was studied by the measurement of turbidity in presence or absence of extract at 620 nm using UV/Vis spectrophotometer. Totalphenol and flavonoid contents were also evaluated. Polyphenol content was found to be more present in the leaves extract (9.29±0.009mg of Gallic acid equivalent (GAE)/g) compared to the flowers extract(5.92±0.14mg GAE/g). Flavonoids content was also found to be more present in the floral extract that is estimated at 0.18±0.01mg QE/g compared to the flowers extract (0.47±0.0035mg QE/g). For the antilithiasis activity, the results clearly shown that P. argentia extracts inhibitedcalcium oxalate crystallization by concentration-dependent manner. The maximum percent inhibition of calcium oxalate by flowers extract was found to be 70.97% at 5mg/mL. Further, P. argentea leaf extract has shown antilithiasic properties and may be used for the prevention of kidneys stones.
Bioactive, Kidney stones, Calcium oxalate, Paronychia argentia, Antilithiasic properties
