1Department of Pharmacy, University of Rajshahi, Bangladesh
2Department of Chemistry, University of Rajshahi, Bangladesh
3Department of Chemistry, Tennessee State University, Nashville, United States
4Department of Pharmacy, Southeast University, Bangladesh
6Professor, Department of Chemistry, Rajshahi University, Bangladesh
*Corresponding Author E-mail: kudrat.chem@ru.ac.bd
Online published on 2 September, 2025.
The investigation developed a new stability-indicating Reverse phase high-pressure liquid chromatographic (RP-HPLC) assay method to quantify Voglibose in bulk and pharmaceutical dosage form. The technique used isocratic elution on an Amino (NH2) column (250x4.6mm, 5µ particle size) with a mobile phase of Phosphate Buffer (pH 6.7) and Acetonitrile (27:73 v/v). The separation was monitored using UV detection at 210 nm. The method was validated, meeting ICH guidelines. The retention time of Voglibose was 6.38 minutes, and the linearity was between 24-56 µg/ml. The limit of detection and quantitation were 0.70 and 2.11 µg/ml, respectively. Analytical performance parameters were determined according to ICH guidelines. The method was simple, precise, accurate, robust, and rapid, making it suitable for quantifying Voglibose in bulk and tablet formulation.
Graphical Abstract:
Reverse Phase HPLC, Voglibose, Method development and validation, ICH Guidelines
