Method development and validation of simethicone and cinitapride by UV- spectroscopy and RP-HPLC in solid dosage forms

A simple, sensitive, accurate, rapid and economical UV Spectrophotometric method was developed and validated of Simethicone and Cinitapride in pure drug and tablet dosage form. The absorbance was measured at 276.8nm using Ether and Dimethylsulfoxide as solvent system. It obeyed Beer’s law at the concentration range of 2-14μg/ml and 2-12μg/ml with coefficient of correlation (r2) of 0.998 and 0.9993. Limit of detection (LOD) was found to be 1.226μg/ml and 1.224μg/ml and Limit of quantitation (LOQ) was found to be 5.226 μg/ml and 4.088μg/ml. The proposed analytical method was validated according to ICH guidelines, yielded good results concerning range, linearity, precision, accuracy, robustness and ruggedness.