Phyto-Physico chemical evaluation, Anti-Inflammatory and Anti microbial activities of Aerial parts of Gmelina asiatica

The present study was carried out to investigate the anti inflammatory, anti bacterial and anti fungal activity of of petroleum ether, chloroform, ethyl acetate and ethanolic extract obtained from Gmelina asiatica. All the extracts were prepared from aerial parts of Gmelina asiatica by hot percolation method in soxhlet apparatus. All extracts of Gmelina asiatica were tested for antibacterial efficacy against Bacillus subtilis, Staphylococcus aureus, Micrococcus luteus, Escherichia coli, Salmonella typhi and pseudomonas aeruginosa and antifungal efficacy against Candida albicans and Aspergillus niger. The Antibacterial and Anti fungal effect produced by petroleum ether, chloroform, ethyl acetate and ethanol extract were comparable to that of Amikacin and Griseofulvin. The Chloroform extract was found to be more effective and showed Anti bacterial and antifungal activity against the entire organism tested. All the extracts at dose concentration (500 mg/kg) were screened for antimicrobial activity. The ethanolic extract of Gmelina asiatica at (250 and 500 mg/kg) concentrations exhibited anti inflammatory activity in carrageenan induced rat paw oedema, histamine induced odema, dextran induced odema and cotton pellet induced granuloma method, and the results are compared to that of standard drug Indomethacin. The results were found to be significiant (P<0.001) when compared to control.