A Simple and Sensitive HPTLC Method for Estimation of Pioglitazone In Bulk and Tablet Dosage Forms

A simple and sensitive, HPTLC method has been developed for the quantitative estimation of pioglitazone in its single component tablet formulations (30 mg). Pioglitazone was chromatographed on silica Gel 60 F₂₅₄ TLC plate using Toluene: methanol: ammonia (7:3:0.1 v/v) as mobile phase. Pioglitazone showed Rf value 0.50±0.03 and scanned at 268 nm using Camag TLC Scanner 3. The method was validated in terms of linearity (200–1200 ng/spot), precision (intra-day variation 0.6 to 3.4%, inter-day variation 0.8 to 3.8%), accuracy (98.7 to 102.5%) and specificity. The limit of detection and limit of quantification for pioglitazone were found to be 5 ng/spot and 20 ng/spot, respectively. The developed method was successfully used for the assay of pioglitazone tablet formulations. The method is simple, sensitive and precise; it can be used for the routine quality control testing of marketed formulations.