Development and Validation of UV Spectrophotometric Method of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation

A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of cefuroxime axetil in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on measurement of absorption at maximum wavelength 281nm.The percentage recovery of cefuroxime axetil ranged from (99.97 ± 0.3969) in pharmaceutical dosage form. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. Beers law was obeyed in the concentration range of 4–28μg/ml having line equation y = 0.0346x + 0.0566 with correlation coefficient of 0.9999. Results of the analysis were validated statistically and by recovery study.