1School of Pharmacy Technology and Management, SVKM's, NMIMS University, Shirpur Campus, Babulde, Near Bank of Tapi River, Shirpur, Dist: Dhule (M.S), India- 425405
2Research and Development Division, Concept Pharmaceuticals Ltd, Aurangabad
*Corresponding Author E-mail: saurabhk77@gmail.com
Online published on 13 March, 2013.
A reverse phase high performance liquid chromatographic method was developed for simultaneous determination of Ceftriaxone Sodium and Sulbactam Sodium in bulk and Pharmaceutical Formulation. The separation was done by a Kromasil C18 column (250 mm × 4.6 mm, 5μm) using Acetonitrile: Buffer PH 7: Distilled Water (400:50:550, v/v/v) as mobile phase. The validation of the method was performed, and specificity, reproducibility, precision and accuracy were confirmed. The limit of detection was approximately 0.39 μg/ml for Ceftriaxone Sodium and 0.08 μg/ml for Sulbactam sodium. Due to simplicity and accuracy the method particularly suitable for routine pharmaceutical quality control.
RP-HPLC, Ceftriaxone Sodium, Sulbactam sodium
