Asian Journal of Research in Chemistry
  • Year: 2009
  • Volume: 2
  • Issue: 4

Purification and Characterization of GroES and GroEL from Genetically Modified Strain E. coli U1/Pus01/Puss1δCat

  • Author:
  • Alok Kumar Verma, GP Pal
  • Total Page Count: 4
  • Page Number: 565 to 568

Deptarment of Molecular Biology, IMS BHU, Varanasi-221005

*Corresponding Author E-mail: alok_bhu2004@rediffmail.com

Online published on 13 March, 2013.

Abstract

In Present study our aim is to purify and characterize GroES and GroEL from genetically modified strain E.coli U1/pUS01/pUSS1δCAT L.GroES was eluted at 0.2M NaCl and GroEL was at 0.3M NaCl. From1.4 litres culture 7.5mg GroEL and 50mg GroES was purified.

Keywords

Cheperonin, GroEL, GroES, IPTG, Q-Sepharose FF, Sephacryl S-200 HR