RP HPLC Determination of Amlodipine in Its Pharmaceutical Dosage Forms

A new and simple reverse phase HPLC method was developed for the determination of amlodipine in its pharmaceutical dosage form. The mobile phase used acetonitrile, methanol, KH₂PO₄ was in the ratio of 250: 250: 500. Buffer solution was prepared by dissolving 0.05m KH₂PO₄ + 0.1% H₃PO₄ adjunct with triethylamine. The separation was achieved on hypersil C-18 column, phenomenex Gemini (250×4.6mm) and 5μ particle size with rheodyne injector. The flow rate was 1ml/min and UV detection at 238nm. The retention time for amlodipine was 9 min. The linearity coefficient of Amlodipine was 0.99% and the percentage recoveries for amlopdipine was 100.02% respectively. The proposed method was accurate, precise and linear within the desired range. This method can be successfully employed for the quantitative analysis of amlodipine.