Development and Validation of UV Spectrophotometric Method of Glibenclamide (Glyburide) in Bulk and Pharmaceutical Formulation

The present research work discussed the development of a UV estimation method for glibenclamide. A simple, accurate, cost effective and reproducible spectrophotometric method has been developed for the estimation of Glibenclamide in bulk and pharmaceutical dosage form. UV spectrophotometric method, which is based on Measurement of absorption at maximum wavelength 242nm.The percentage recovery of Glibenclamide ranged from (99.97 ± 0.3969) in pharmaceutical dosage form. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. Beers law was obeyed in the concentration range of 5–30 μg/ml having line equation y = 0.0211x -0.0114 with correlation coefficient of 0.9934. Results of the analysis were validated statistically and by recovery study.